Biosafety cabinet – Made in 1909, the first biological safety cabinets were characterized as microbiological isolation boxes. The pre-filtered air was drawn through the booth which was maintained under negative pressure. Thus was then exhausted through a flask containing disinfectant. The first cabin biosecurity “modern” was developed by Van den Ended in 1943. A gas nozzle in the exhaust duct creating a circular movement of the air inside the cabin and incinerated exhaustion.
The project has been improved, and by 1953, the predecessor of the current cabin class I was in use, albeit with low efficiency exhaust filters. The exhaust air had to be incinerated and the recalculated air was inconceivable. In 1962 HEPA filters were applied. This allowed the filtered air could be exhausted directly outside the laboratory or recirculated, leading to the establishment of the three classes of currently existing cabins. Although seemingly simple equipment, biosafety cabinet have built a complex technology.
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Materials and constructiveness, use and decontamination procedures, performance testing at the factory and in the field are some of the issues that were not dealt with here. The NSF- 49 is a recommended reference. Finally warn that a biosafety cabinet is a device that interacts with the site where it is installed and should therefore be integrated into the concept of building air handling project and be part of the installation qualification processes.
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